Thesis on antioxidant activity of medicinal plants

Phytochemical screening of TLM revealed the presence of alkaloids, anthraquinones, cardiac glycosides, coumarins, flavonoids, saponins, phlobatannins, tannins and terpenoids.

Antimicrobial activity of medicinal plants phd thesis

Reference chemicals were used for comparison in all assays. The residue was suspended in water 50 ml and partitioned successively with n-hexane, chloroform, ethyl acetate, n-butanol a total of two aliquots of ml each and soluble residual aqueous fraction yielding respectively the TLH 5. Most plants screened showed some inhibitory activity with either or both acetyl- and butyrylcholinesterase. Although the toxicity profile of most medicinal plants have not been thoroughly evaluated, it is generally accepted that medicines derived from plant products are safer than their synthetic counterparts [ 3 , 4 ]. Natural antioxidants either in the form of raw extracts or their chemical constituents are very effective to prevent the destructive processes caused by oxidative stress [ 2 ]. Digestion is an initial step involving changes in pH and activity of proteolytic enzymes. Then the absorbance was taken at nm. The evidence has brought the attention of scientists to an appreciation of antioxidants for prevention and treatment of diseases, and maintenance of human health [ 5 ]. Antioxidant agents of natural origin have attracted special interest because of their free radical scavenging abilities [ 12 ]. Results The total phenolic contents TPC A selection of ten Libyan plants which have various ethnobotanical uses were evaluated for anticholinesterase activity. The scavenging activity was estimated based on the percentage of DPPH radical scavenged as the following equation: Scavenging. It is represented in Iran by nine species [ 15 ]. Most extracts showed an increase in antioxidant activity after the final pancreatin step although results varied with the antioxidant assay used.

Substantial evidence has accumulated and indicated key roles for reactive oxygen species ROS and other oxidants in causing numerous disorders and diseases. The role of free radical reactions in disease pathology is well established and is known to be involved in many acute and chronic disorders in human beings, such as diabetes, atherosclerosis, aging, immunosuppression and neurodegeneration [ 11 ].

Extract preparation The fresh, whole plant 3 kg was collected and shade dried to obtain g dry sample which was later coarsely powdered in a Willy Mill to mesh size and used for solvent extraction.

Thesis on antioxidant activity of medicinal plants

It is suggested that the efficacy of these plants could be explained, at least in part, by their antioxidant activity. The residue was suspended in water 50 ml and partitioned successively with n-hexane, chloroform, ethyl acetate, n-butanol a total of two aliquots of ml each and soluble residual aqueous fraction yielding respectively the TLH 5. Substantial evidence has accumulated and indicated key roles for reactive oxygen species ROS and other oxidants in causing numerous disorders and diseases. Antioxidants stabilize or deactivate free radicals, often before they attack targets in biological cells [ 8 ]. Despite the great advances observed in modern medicine in recent decades, plants still make an important contribution to health care. Most extracts showed an increase in antioxidant activity after the final pancreatin step although results varied with the antioxidant assay used. Testing in vitro bioavailability of plant extracts is a useful step in evaluating in vivo bioavailability. Recently interest in naturally occurring antioxidants has considerably increased for use in food, cosmetic and pharmaceutical products, because they possess multifacetedness in their multitude and magnitude of activity and provide enormous scope in correcting imbalance [ 9 , 10 ]. The evidence has brought the attention of scientists to an appreciation of antioxidants for prevention and treatment of diseases, and maintenance of human health [ 5 ]. These methods enable high-throughput screening of potential antioxidant capacity.

Osaka, Japan. The plant material was botanically identified by Dr. A selection of ten Libyan plants which have various ethnobotanical uses were evaluated for anticholinesterase activity.

In vitro antioxidant activity thesis

Results show that of these twenty three plants, hot and cold extracts of Myrtus communis, Quercus robur and Syzygium aromaticum exhibited the strongest antioxidant activity in all tests and this is higher than that of the green tea control. Most plants screened showed some inhibitory activity with either or both acetyl- and butyrylcholinesterase. Most extracts showed an increase in antioxidant activity after the final pancreatin step although results varied with the antioxidant assay used. These methods enable high-throughput screening of potential antioxidant capacity. Results The total phenolic contents TPC The scavenging activity was estimated based on the percentage of DPPH radical scavenged as the following equation: Scavenging. Extract preparation The fresh, whole plant 3 kg was collected and shade dried to obtain g dry sample which was later coarsely powdered in a Willy Mill to mesh size and used for solvent extraction. Then the absorbance was taken at nm. It is not known which constituents of plant are associated in reducing the risk of chronic diseases, but antioxidants appear to play a major role in the protective effect of plant medicine. Studies on herbal plants, vegetables, and fruits have indicated the presence of antioxidants such as phenolics, flavonoids, tannins, and proanthocyanidins. The search for novel natural antioxidants of plant origin has ever since increased. Plant extracts were evaluated for possible changes in antioxidant properties and anticholinesterase activity using an artificial digestion technique. Distilled deionized water dd. The control was prepared as above without any sample.

The use of medicinal plants with high level of antioxidant constituents has been proposed as an effective therapeutic approach for hepatic damages [ 13 ]. Results show that of these twenty three plants, hot and cold extracts of Myrtus communis, Quercus robur and Syzygium aromaticum exhibited the strongest antioxidant activity in all tests and this is higher than that of the green tea control.

It is well known that free radicals cause cell damage through mechanisms of covalent binding and lipid peroxidation with subsequent tissue injury. Antioxidants stabilize or deactivate free radicals, often before they attack targets in biological cells [ 8 ].

Testing in vitro bioavailability of plant extracts is a useful step in evaluating in vivo bioavailability. Torilis leptophylla belonging to the Apiaceae family [ 14 ] or more commonly known as Bristle fruit Hedge parsley, is a forb of the genus Torilis, distributed in Asia, Europe and North Africa.

The ingestion of natural antioxidants has been inversely associated with morbidity and mortality from degenerative disorders [ 6 ].

antioxidant activity of plant extracts

Although the toxicity profile of most medicinal plants have not been thoroughly evaluated, it is generally accepted that medicines derived from plant products are safer than their synthetic counterparts [ 34 ].

Rated 9/10 based on 46 review
Download